Columbia Science Review
  • Home
  • About
    • Executive Board
    • Editorial Board
  • Blog
  • Events
    • 2022-2023
    • 2021-2022
    • 2020-2021
    • 2019-2020
    • 2018-2019
    • 2017-2018
    • 2016-2017
  • Publications
  • COVID-19 Public Hub
    • Interviews >
      • Biology of COVID-19
      • Public Health
      • Technology & Data
    • Frontline Stories >
      • Healthcare Workers
      • Global Health
      • Volunteer Efforts
    • Resources & Links >
      • FAQ's
      • Resource Hubs
      • Student Opportunities
      • Podcasts & Graphics
      • Mental Health Resources
      • Twitter Feeds
      • BLM Resources
    • Columbia Events >
      • Campus Events
      • CUMC COVID-19 Symposium
      • CSR Events
    • Our Team
  • Contact

A Short Primer on CRISPR Technology and the Age of Genetic Engineering

4/22/2014

0 Comments

 
Picture
By Aditya Nair

A clever modification to a bacterial immune response could hold the key to curing hundreds of genetic diseases.

We humans, from our selfish point of view, are accustomed to considering bacteria as agents of infection and the causes of great suffering. The all too common “strep throat” is the result of infection by a family of bacteria known as streptococcus. Clostridium Botulinum produces the strongest known toxin in the world. Some of the more active members of society may be familiar with staph infection, the scourge of athletes and athletic trainers nationwide. It could even be argued that the very course of human history has been altered by bacteria; the bubonic plague (Yersinia pestis) is a bacterial disease.

However, bacteria do not enjoy a “free ride” biologically. They are not merely parasites limited by the number of hosts that they can infect. They, just like us, have natural predators. For example, a bacteriophage is a virus that infects bacteria. In order to replicate, a bacteriophage must insert its genetic material into the bacteria; then, the bacteriophage’s DNA can be incorporated into the bacteria’s own DNA. The bacteriophage genetic material causes the bacteria to change its cellular processes to begin producing more of the bacteriophage.

Humans have elaborate immune systems that deal with this sort of threat, and as it turns out, bacteria may be equipped with them too. CRISPRs (clustered regularly interspaced short palindromic repeats) are sections of DNA in bacteria that code for and work with proteins called Cas (CRISPR-associated) proteins that can inactivate foreign DNA such as that inserted by a bacteriophage. In the process, the CRISPR system incorporates a section of the foreign DNA into its own genetics, which helps it recognize and inactivate foreign DNA during future infections.

The fact that bacteria were able to selectively modify their own DNA was of great interest to scientists. The implications became clear very quickly. Could it be possible to re-engineer this system so that any given sequence of DNA could be inserted into bacterial genomes? Could this system work in eukaryotes or even mammals? Could this system be used to cure genetic diseases?

The answer to all of these questions is yes.

CRISPR immunity works by recognizing particular sequences of DNA, and using those sequences as guidance to know when to make cuts. The section of DNA that is cut out can be replaced with new DNA, or can merely be deleted, with the cut ends coming back together. By re-engineering the sequences that the CRISPR system recognizes, scientists can cut almost anywhere in the genome, and replace the excised section with another one.

This is an ability that scientists have had for many years. However, previous approaches often required the killing of cells, laboratory isolation of DNA, and a rather difficult and roundabout process to re-incorporate DNA into a limited range of possible living beings.

However, CRISPR can work while an animal is still living, and there’s incredible evidence that the biological systems allowing it to work in bacteria will also work in mammals.

Amazingly, in March, a team from MIT was successful in correcting a rare genetic liver disorder in mice using the system, altering the very genome of the mouse to cure the disease.

We may very well be witnessing the birth of a revolution in genetic medicine, equipped with the ability to delete faulty genes and substitute them with healthy versions. Genetic disorders could soon be relics of the past, and the age of genetic engineering may well be upon us.
​
And who would have thought that the keys to this technology lay within our sworn enemies, the lowly bacteria.
0 Comments



Leave a Reply.

    Categories

    All
    Artificial Intelligence
    Halloween 2022

    Archives

    November 2022
    October 2022
    June 2022
    January 2022
    May 2021
    April 2021
    March 2021
    February 2021
    January 2021
    December 2020
    November 2020
    October 2020
    September 2020
    August 2020
    July 2020
    June 2020
    May 2020
    April 2020
    March 2020
    February 2020
    January 2020
    November 2019
    October 2019
    April 2019
    March 2019
    February 2019
    January 2019
    December 2018
    November 2018
    October 2018
    April 2018
    March 2018
    February 2018
    November 2017
    October 2017
    May 2017
    April 2017
    April 2016
    March 2016
    February 2016
    December 2015
    November 2015
    October 2015
    May 2015
    April 2015
    March 2015
    February 2015
    January 2015
    December 2014
    November 2014
    October 2014
    May 2014
    April 2014
    March 2014
    February 2014
    December 2013
    November 2013
    October 2013
    April 2013
    March 2013
    February 2013
    January 2013
    December 2012
    November 2012
    October 2012
    April 2011
    March 2011
    February 2011
    September 2010
    August 2010
    July 2010
    June 2010
    May 2010
    April 2010
    March 2010
    February 2010
    January 2010
    December 2009
    November 2009
    July 2009
    May 2009

Columbia Science Review
© COPYRIGHT 2022. ALL RIGHTS RESERVED.
Photos used under Creative Commons from driver Photographer, BrevisPhotography, digitalbob8, Rennett Stowe, Kristine Paulus
  • Home
  • About
    • Executive Board
    • Editorial Board
  • Blog
  • Events
    • 2022-2023
    • 2021-2022
    • 2020-2021
    • 2019-2020
    • 2018-2019
    • 2017-2018
    • 2016-2017
  • Publications
  • COVID-19 Public Hub
    • Interviews >
      • Biology of COVID-19
      • Public Health
      • Technology & Data
    • Frontline Stories >
      • Healthcare Workers
      • Global Health
      • Volunteer Efforts
    • Resources & Links >
      • FAQ's
      • Resource Hubs
      • Student Opportunities
      • Podcasts & Graphics
      • Mental Health Resources
      • Twitter Feeds
      • BLM Resources
    • Columbia Events >
      • Campus Events
      • CUMC COVID-19 Symposium
      • CSR Events
    • Our Team
  • Contact